VDJGermline is a bioinformatics-based tool for antibody sequence VDJ analysis. Through sequence alignment, it can quickly retrieve the most homologous sequences to the target antibody sequences from a rich VDJ gene library, and deeply analyze the VDJ gene rearrangement information. It is particularly suitable for the sequence analysis of animal immune repertoires. The gene library covers multiple species, including humans, mice, rhesus monkeys, and camels, and has specifically constructed the “HUGO H3K3” fully humanized mouse gene library. It outputs analysis result tables for each sequence, visual alignment charts, and a variety of statistical analysis charts.

ImmuneBuilder, including ABodyBuilder2, NanoBodyBuilder2, and TCRBuilder2, is specifically designed for predicting the structure of immunoproteins (e.g., antibodies, nanobodies, and T-cell receptors), and employs AlphaFold-Multimer's structural modules with modifications specific to the immunoproteins to improve prediction accuracy.ImmuneBuilder is able to quickly generate immunoprotein structures that resemble experimental data much faster than AlphaFold2 and without the need for large sequence databases or multiple sequence comparisons. The tool's features include high accuracy, fast prediction, and open-source accessibility for structural analysis of large-scale sequence datasets, especially in the study of immunoprotein structures from next-generation sequencing data. immuneBuilder also provides error estimation to help filter out erroneous models, enhancing its value for applications in biotherapeutics and immunology research. Figure 1 shows the architecture of AbBuilder2, and the same architecture is used for NanoBodyBuilder2 and TCRBuilder2.